By Carlos L Céspedes; et al
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Annual Review of Pharmacology and Toxicology 44, 239–267. L. & Etherton, T. , 2004. Bioactive compounds in nutrition and health-research methodologies for establishing biological function: The antioxidant and anti-inflammatory effects of flavonoids on atherosclerosis. Annual Review of Nutrition 24, 511–538. , 2000. Introduction: aging research comes of age. Cellular and Molecular Life Sciences 57, 695–697. , 2001. Disease model: human aging. Trends in Molecular Medicine 7, 179–181. , 2007. Suppression of aging in mice by the hormone klotho.
Because such production is strongly linked with MPO released by neutrophils, often it must be reinforced with HRP, because in the first steps of the respiratory burst the amount of MPO in the extracellular environment is minimal. As mentioned below, it must be remembered that the probes used are not always selective enough. Thus, both luminol and isoluminol preferably detected HOCl, showing less sensitivity for the case of superoxide anion and hydrogen peroxide. When looking for a detailed study of the effect on NADPH oxidase activity, and therefore on superoxide anion production, the alternative use of lucigenin is more recommend able.
Indeed, their response to H2O2 is 500 times greater than for other ROS. A family of boronate probes has been synthesized, such as peroxyresofurin-1 (PR-1), greenfluorescent PF-1 and the blue-fluorescent peroxyxanthone-1 (PX1). Interestingly, all these probes are permeable and can detect New probes for hypochlorous acid sulfonaphthoaminophenyl fluorescein (snapf). SNAPF is a newly developed fluoresceinderived probe for the specific detection of intracellular levels of HOCl generated by MPO activity.