By Edward A. Burton, Shaohua Huang, William F. Goins (auth.), Curtis A. Machida (eds.)
The promise of gene remedy may be learned provided that doable vectors are available to bring healing genes. In Viral Vectors for Gene treatment: equipment and Protocols, major researchers from academia and biotechnology describe confirmed molecular tools for the development, improvement, and use of virus vectors for gene move and gene treatment. providing specific step by step directions to make sure winning effects, those specialists element using herpes viruses, adenoviruses, adeno-associated viruses, uncomplicated and intricate retroviruses, together with lentiviruses, and different virus platforms for vector improvement and gene move. extra chapters display using virus vectors within the mind and vital anxious process. every one protocol contains a dialogue of the foundations concerned, quite a few charts and tables, considerable references, and notes on attainable difficulties, troubleshooting, and replacement strategies.
finished and hugely sensible, Viral Vectors for Gene treatment: tools and Protocols offers not just researchers with the elemental instruments had to layout exact gene supply vectors, but additionally clinicians with an knowing of the way to use viral vectors to the therapy of genetic disorders.
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Extra resources for Viral Vectors for Gene Therapy: Methods and Protocols
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20. Harvest virus and carry out a further round of limiting dilution plaque isolation, as in steps 15–19. 21. 6. 3. Titration of Virus Stock 1. Prepare a series of tenfold dilutions (10–2 to 10–10) of the virus stock in 1 mL of MEM without serum. 2. 5 × 106 cells in 1 mL MEM. 3. Incubate at 37°C for 1 h on a Nutator rocker platform. 4. Plate the cells in six-well plates and incubate the plates at 37°C in a CO2 incubator overnight. 5. 5 mL of 1% methylcellulose, 5% FBS in MEM. 6. Incubate the plates for 3–5 d until well-deﬁned plaques appear.
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